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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Na2-EDTA Effects on the Development of Oyster, Crassostrea belcheri (Sowerby) Larvae) ผู้เขียน: Suwat Tanyaros สื่อสิ่งพิมพ์:pdf AbstractThe effect of different concentrations of a disodium salt of ethylenediamine tetraacetic acid (Na2-EDTA) on the development of oyster Crassostrea belcheri, was studied and the highest percentage of fertilized eggs that developed into normal trochophore larvae was found to occur in seawater untreated with Na2-EDTA. However, no signifi cant differences in mean percentages of embryos that developed into normal D-larvae were found among untreated and treated units at Na2-EDTA concentrations ranging from 5 to 20 ppm (P > 0.05). In the study on the effect of conditioning periods of Na2-EDTA pre-treated seawater on the development of oyster larvae, non-signifi cant differences were found among the mean percentages of fertilized eggs that developed into normal trochophore larvae in untreated seawater and seawater pre-treated with Na2-EDTA and conditioned from 0 to 48 hr (P > 0.05). However, mean percentages of embryos that developed into normal D-larvae was highly signifi cantly different among treatments (P < 0.01), with the highest percentage occurring in untreated Na2-EDTA seawater. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Nursing and Grow-Out of Hatchery-Reared Big Oyster (Crassostrea belcheri Sowerby 1871) in the Intertidal Mangrove Area) ผู้เขียน:Suwat Tanyaros, Kunchai Anan, Lawrence D. Kitt สื่อสิ่งพิมพ์:pdf AbstractThe nursing of hatchery-reared juvenile oysters (Crassostrea belcheri Sowerby 1871) in the intertidal mangrove area was carried out over four months. The initial average width and length of the seed were both 2?0.05 mm, while average seed weight was 0.29?0.05 g. At the end of the experiment, the oyster seed reached 3.38?0.39 and 3.54?0.89 cm for average width and length, respectively and weighed an average of 3.22?2.01 g. The mean increase in daily growth rate of the shell width and length ranged from 0.13 to 0.29 and 0.12 to 0.38 mm/individual, respectively. The mean increase in live weight ranged from 0.16-0.39 g/individual. The mean survival rate was 22.52% at the end of the nursing experiment. For the grow-out experiment, oysters from the nursing experiment which were five cm or bigger were chosen and placed in flipping pouches in the intertidal zone for six months. At the end of this grow-out experiment, the mean increases in shell width and length were not significantly different among the treatments (p>0.05). However, there were significant differences in survival and in the amount of barnacle attachment among the treatments (p<0.05). A high survival rate was found with all densities after a five hour exposure to air at low tide during the spring tide period. The highest number of attached barnacles was found after an exposure to air of one hr at the lowest tide during spring tide. |
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ที่มา:การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 48: สาขาประมงหัวเรื่อง:ความไวต่อรังสีของแบคทีเรียก่อโรคในหอยนางรม (Crassostrea belcheri) และอาหารเลี้ยงเชื้้อ |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : The Effect of Substrate Conditioning on Larval Settlement and Spat Growth of the Cupped Oyster, Crassostrea belcheri (Sowerby), in a Hatchery) ผู้เขียน:Suwat Tanyaros สื่อสิ่งพิมพ์:pdf AbstractSubstrate conditioning is a key factor for the settlement and metamorphosis of oyster larvae. The effects of substrate conditioning on larval settlement and growth of spat of the cupped oyster, Crassostrea belcheri, were evaluated in three trials in a closed recirculation system. Particles prepared by grinding clean oyster shell were used as a substrate for larval settlement. In each trial, substrates were conditioned by immersion in seawater for 2, 12 and 24 h before use. The number of larvae that settled on substrates conditioned for 2 h was significantly higher (P < 0.05) than the number that settled on substrates conditioned for 12 and 24 h in all trials. Mean setting rates ranged from 30.44 ? 9.71 to 61.71 ? 11.28, 7.61 ? 3.51 to 35.77 ? 1.67 and 6.21 ? 1.38 to 32.77 ? 1.57 on substrates conditioned for 2, 12 and 24 h, respectively. Differences in the increments of shell width and length among oyster spat nursed over 3 wk on substrate conditions for different lengths of time were nonsignificant (P > 0.05). |
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ที่มา:Kasetsart Journal (Natural Science)(วารสารวิทยาสารเกษตรศาสตร์ สาขาวิทยาศาสตร์)หัวเรื่อง:Characterization of antibacterial protein from hemolymph of oyster Crassostrea belcheri |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characterization of Antibacterial Protein from Hemolymph
of Oyster Crassostrea belcheri) ผู้เขียน:วิราวรรณ จุลโพธิ์ (นุชนารถ),  ดร.สุภาวดี พุ่มพวง, ศาสตราจารย์ , ดร.สุริยัน ธัญกิจจานุกิจ, ผู้ช่วยศาสตราจารย์ , ดร.สุตเขตต์ นาคะเสถียร, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe hemolymph of oyster Crassostrea belcheri was found to possess significant antibacterial
activity against Vibrio harveyi, V. vulnificus and V. cholerae. Hemolymph was partially purified by gel
filtration chromatography on Sephacryl S-200 and fractioned into four parts: P1, P2, P3 and P4. The P3
showed the highest antibacterial activity against V. parahaemolyticus, V. cholerae, V. alginolyticus, V.
harveyi in the inhibition ranges from 95-62% and V. vulnificus was inhibited only 8% with 64 mg
protein. The antibacterial activity of P3 protein was stabilized at pH 6-8 and 4-30?C. In addition, the
activity required calcium ion. Molecular weight of the antibacterial protein P3 determined by gel filtration
was approximately 18.7 kDa. Antibacterial protein gave two bands of 25.0 and 30.5 kDa in SDS-PAGE
and pI was 3.0 and 5.0, respectively. Both bands were analyzed by LC-MS/MS and compared with
nrFasta database. The 30.5 kDa band was homologous to hemocyte extracellular superoxide dismutase
from C. gigas and 25.0 kDa band was homologous to sarcoplasmic calcium-binding protein. |
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